Issue 98, 2021
From the journal:

Chemical Communications

Design of a surrogate for high throughput screening of fatty aldehyde reductase engineering†

Suphanida Worakaensai,a   Surayut Kluaiphanngam,a   Sirawit Wet-osot, ORCID logo a   Ratana Charoenwattanasatien, ORCID logo bc   Utumporn Ngivprom,a   Chuthamat Duangkamol,a   Anyanee Kamkaew ORCID logo a  and  Rung-Yi Lai ORCID logo *ac  

* Corresponding authors

a School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000 Thailand
E-mail: rylai@sut.ac.th

b Synchrotron Light Research Institute (Public Organization), 111 University Avenue, Nakhon Ratchasima, 30000 Thailand

c Center for Biomolecular Structure, Function and Application, Suranaree University of Technology, Nakhon Ratchasima, 30000 Thailand

Abstract

We designed and synthesized a fatty aldehyde surrogate containing a formyl thioester group, which can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay. We demonstrated its successful application in the high throughput screening of FALR engineering.

Graphical abstract: Design of a surrogate for high throughput screening of fatty aldehyde reductase engineering

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Article information

DOI
https://doi.org/10.1039/D1CC05472D
Article type
Communication
Submitted
28 Sep 2021
Accepted
02 Nov 2021
First published
09 Nov 2021

Chem. Commun., 2021,57, 13373-13376

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Design of a surrogate for high throughput screening of fatty aldehyde reductase engineering

S. Worakaensai, S. Kluaiphanngam, S. Wet-osot, R. Charoenwattanasatien, U. Ngivprom, C. Duangkamol, A. Kamkaew and R. Lai, Chem. Commun., 2021, 57, 13373 DOI: 10.1039/D1CC05472D

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