G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p

Jun Gao; Zhaofeng Gao; Andrea A. Putnam; Alicia K. Byrd; Sarah L. Venus; John C. Marecki; Andrea D. Edwards; Haley M. Lowe; Eckhard Jankowsky; Kevin D. Raney

doi:10.1039/D1CC01479J

G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p†

Jun Gao,

‡^a Zhaofeng Gao,‡^b Andrea A. Putnam,‡^b Alicia K. Byrd,

^a Sarah L. Venus,^b John C. Marecki,^a Andrea D. Edwards,^a Haley M. Lowe,^a Eckhard Jankowsky*^b and Kevin D. Raney*^a

Author affiliations

* Corresponding authors

^a Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
E-mail: RaneyKevinD@uams.edu

^b Center for RNA Science and Therapeutics, Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
E-mail: exj13@case.edu

Abstract

G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid–liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains.

Chemical Communications

G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p†

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G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p

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