Issue 31, 2021
From the journal:

Chemical Communications

Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging†

Yingjie Yu,‡a   Lidan Li, ORCID logo ‡b   Guobao Li,b   Xu Zhou,c   Tingting Deng,a   Meng Liang*c  and  Guohui Nie*a  

* Corresponding authors

a Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China
E-mail: nieguohui@email.szu.edu.cn

b National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China

c College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
E-mail: liangmeng@mail.buct.edu.cn

Abstract

We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.

Graphical abstract: Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D1CC00536G
Article type
Communication
Submitted
29 Jan 2021
Accepted
12 Mar 2021
First published
12 Mar 2021

Chem. Commun., 2021,57, 3753-3756

Permissions

Request permissions

Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging

Y. Yu, L. Li, G. Li, X. Zhou, T. Deng, M. Liang and G. Nie, Chem. Commun., 2021, 57, 3753 DOI: 10.1039/D1CC00536G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements