Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations

Shiliang He; Mengmeng Liu; Fangfei Yin; Jiangbo Liu; Zhilei Ge; Fan Li; Min Li; Jiye Shi; Lihua Wang; Xiuhai Mao; Xiaolei Zuo; Qian Li

doi:10.1039/D1CC00266J

Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations†

Shiliang He,‡^a Mengmeng Liu,‡^b Fangfei Yin,^ac Jiangbo Liu,^a Zhilei Ge,^d Fan Li,^a Min Li,^a Jiye Shi,^c Lihua Wang,

^c Xiuhai Mao,

^a Xiaolei Zuo

^a and Qian Li

*^d

Author affiliations

* Corresponding authors

^a Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

^b Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

^c Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

^d School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China
E-mail: liqian2018@sjtu.edu.cn

Abstract

The response sensitivity of a molecular sensor is determined by the folding cooperativity of its responsive module. Using an H⁺-responsive dimeric DNA i-motif as a model, we demonstrate the enhancement of its folding cooperativity through preorganization by a DNA framework, and with it we fabricate robust intracellular pH sensors with high response sensitivity.

Chemical Communications

Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations†

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Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations

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