Issue 21, 2021
From the journal:

Chemical Communications

Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro†

Joongoo Lee, ORCID logo ‡a   Kevin J. Schwarz,‡b   Hao Yu,c   Antje Krüger,a   Eric V. Anslyn, ORCID logo d   Andrew D. Ellington,e   Jeffrey S. Moore*bf  and  Michael C. Jewett ORCID logo *a  

* Corresponding authors

a Department of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern University, Evanston, IL 60208, USA

b Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA
E-mail: jsmoore@illinois.edu

c Departments of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA

d Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA

e Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA

f Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA

Abstract

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.

Graphical abstract: Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D0CC07740B
Article type
Communication
Submitted
25 Nov 2020
Accepted
22 Jan 2021
First published
22 Jan 2021

Chem. Commun., 2021,57, 2661-2664

Author version available


Download author version (PDF)

Permissions

Request permissions

Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

J. Lee, K. J. Schwarz, H. Yu, A. Krüger, E. V. Anslyn, A. D. Ellington, J. S. Moore and M. C. Jewett, Chem. Commun., 2021, 57, 2661 DOI: 10.1039/D0CC07740B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements