A Broccoli aptamer chimera yields a fluorescent K+ sensor spanning physiological concentrations

Abstract

The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K⁺ >30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K⁺ concentrations, yielding an effective RNA-based fluorescent K⁺ sensor.