Issue 5, 2021

Cell-free riboswitches


The emerging community of cell-free synthetic biology aspires to build complex biochemical and genetic systems with functions that mimic or even exceed those in living cells. To achieve such functions, cell-free systems must be able to sense and respond to the complex chemical signals within and outside the system. Cell-free riboswitches can detect chemical signals via RNA–ligand interaction and respond by regulating protein synthesis in cell-free protein synthesis systems. In this article, we review synthetic cell-free riboswitches that function in both prokaryotic and eukaryotic cell-free systems reported to date to provide a current perspective on the state of cell-free riboswitch technologies and their limitations.

Graphical abstract: Cell-free riboswitches

Article information

Article type
Review Article
29 Jun 2021
26 Jul 2021
First published
04 Aug 2021
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2021,2, 1430-1440

Cell-free riboswitches

T. Tabuchi and Y. Yokobayashi, RSC Chem. Biol., 2021, 2, 1430 DOI: 10.1039/D1CB00138H

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