Issue 4, 2021

A self-labeling protein based on the small ultra-red fluorescent protein, smURFP

Abstract

Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.

Graphical abstract: A self-labeling protein based on the small ultra-red fluorescent protein, smURFP

Supplementary files

Article information

Article type
Communication
Submitted
07 Jun 2021
Accepted
24 Jun 2021
First published
29 Jun 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 1221-1226

A self-labeling protein based on the small ultra-red fluorescent protein, smURFP

J. Machado, R. Ting, J. Y. Lin and E. A. Rodriguez, RSC Chem. Biol., 2021, 2, 1221 DOI: 10.1039/D1CB00127B

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