Issue 1, 2021

A two-step resin based approach to reveal survivin-selective fluorescent probes

Abstract

The identification of modulators for proteins without assayable biochemical activity remains a challenge in chemical biology. The presented approach adapts a high-throughput fluorescence binding assay and functional chromatography, two protein-resin technologies, enabling the discovery and isolation of fluorescent natural product probes that target proteins independently of biochemical function. The resulting probes also suggest targetable pockets for lead discovery. Using human survivin as a model, we demonstrate this method with the discovery of members of the prodiginine family as fluorescent probes to the cancer target survivin.

Graphical abstract: A two-step resin based approach to reveal survivin-selective fluorescent probes

Supplementary files

Article information

Article type
Communication
Submitted
11 Jul 2020
Accepted
07 Nov 2020
First published
27 Nov 2020
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2021,2, 181-186

A two-step resin based approach to reveal survivin-selective fluorescent probes

A. J. Ambrose, N. T. Pham, J. Sivinski, L. Guimarães, N. Mollasalehi, P. Jimenez, M. A. Abad, A. A. Jeyaprakash, S. Shave, L. V. Costa-Lotufo, J. J. La Clair, M. Auer and E. Chapman, RSC Chem. Biol., 2021, 2, 181 DOI: 10.1039/D0CB00122H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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