Isolation and characterization of porcine macrophages and their inflammatory and fusion responses in different stiffness environments

Abstract

Evaluating the host immune response to biomaterials is an essential step in the development of medical devices and tissue engineering strategies. To aid in this process, in vitro studies, whereby immune cells such as macrophages are cultured on biomaterials, can often expedite high throughput testing of many materials prior to implantation. While most studies to date utilize murine or human cells, the use of porcine macrophages has been less well described, despite the prevalent use of porcine models in medical device and tissue engineering development. In this study, we describe the isolation and characterization of porcine bone marrow- and peripheral blood-derived macrophages, and their interactions with biomaterials. We confirmed the expression of the macrophage surface markers CD68 and F4/80 and characterized the porcine macrophage response to the inflammatory stimulus, bacterial lipopolysaccharide. Finally, we investigated the inflammatory and fusion response of porcine macrophages cultured on different stiffness hydrogels, and we found that stiffer hydrogels enhanced inflammatory activation by more than two-fold and promoted fusion to form foreign body giant cells. Together, this study establishes the use of porcine macrophages in biomaterial testing and reveals a stiffness-dependent effect on biomaterial-induced giant cell formation.

Graphical abstract: Isolation and characterization of porcine macrophages and their inflammatory and fusion responses in different stiffness environments

Supplementary files

Article information

Article type
Paper
Submitted
14 May 2021
Accepted
26 Aug 2021
First published
02 Sep 2021

Biomater. Sci., 2021, Advance Article

Isolation and characterization of porcine macrophages and their inflammatory and fusion responses in different stiffness environments

V. S. Meli, R. P. Donahue, J. M. Link, J. C. Hu, K. A. Athanasiou and W. F. Liu, Biomater. Sci., 2021, Advance Article , DOI: 10.1039/D1BM00746G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements