A lysosomal targeted fluorescent probe based on coumarin for monitoring hydrazine in living cells with high performance

Abstract

A lysosomal targeted fluorescent probe based on coumarin for monitoring hydrazine (N₂H₄) in living cells was designed and synthesised. The novel fluorescent probe Cou-Lyso-N₂H₄, in response to N₂H₄, exhibited good selectivity, low cytotoxicity, and lysosomal localization, which could be suitable for studying the harmfulness of N₂H₄ in subcellular organelles during various physiological processes.