A phenothiazine-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in food, live cells and animals

Abstract

In this work, a phenothiazine-based fluorescent probe (PR) has been developed for the selective detection of hydrogen sulfide (H₂S) in biosystems and monitoring H₂S produced in the food spoilage process. The nucleophilic attack of H₂S on the CC double bond of PRvia a Michael addition interdicted the ICT process to trigger 34-fold enhancement of the fluorescence emission. PR featured high selectivity and sensitivity (1.8 μM), low cytotoxicity and reliability at physiological pH. “Naked-eye” monitoring of H₂S produced in the food spoilage process using PR was successfully accomplished. The preliminary fluorescence imaging studies showed that PR is suitable for the visualization of exogenous and endogenous H₂S in living cells and live animals. Moreover, PR has been successfully applied to the visualization of H₂S generation in an inflammation model. The results indicated that PR is an effective tool to monitor H₂S production in the fields of biomedicine and food safety.