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Issue 4, 2021

Protein-templated gold nanoclusters as specific bio-imaging probes for the detection of Hg(ii) ions in in vivo and in vitro systems: discriminating between MDA-MB-231 and MCF10A cells

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Abstract

Gold nanoclusters (AuNCs) synthesized within a protein (Human Serum Albumin, HSA) template exhibited intense red luminescence accompanied by a quantum yield >10% and remarkable photo and cluster-core stability for a prolonged period (more than a year). These photoluminescent nanoclusters (NCs) were resistant to chemical and thermal perturbations but break down selectively and highly sensitively in the presence of mercury, Hg(II), ions. The AuNCs were efficient in quantifying Hg(II) ions in solution as well as bound to the hormone insulin. By exploiting the auto-fluorescence of these AuNCs, we demonstrated that our AuNCs were able to sense Hg(II) ions at single-molecule sensitivity using Fluorescence Correlation Spectroscopy (FCS), highlighting a detection limit in the sub-nanomolar regime. The translational diffusion time of the AuNCs decreased significantly upon the interaction with Hg(II) ions and resulted in the formation of smaller sized clusters. A cell viability study reveals the non-toxic nature of these nano-probes, which thus can be used for cell imaging. Interestingly, a cell line-based study reveals that the fluorescence intensity of AuNCs could be detected in cancerous MDA-MB-231 cells but not in non-cancerous breast-derived MCF10A cells. Further, time lapse fixed cell imaging by confocal microscopy revealed that the fluorescence of AuNCs could be quenched by Hg(II) ions inside the MDA-MB-231 cells. Thus the objective of our study is to appraise the sensitive in vivo as well as in vitro detection of Hg(II) ions using AuNCs as a probe.

Graphical abstract: Protein-templated gold nanoclusters as specific bio-imaging probes for the detection of Hg(ii) ions in in vivo and in vitro systems: discriminating between MDA-MB-231 and MCF10A cells

Supplementary files

Article information


Submitted
24 Oct 2020
Accepted
02 Dec 2020
First published
09 Dec 2020

Analyst, 2021,146, 1455-1463
Article type
Paper

Protein-templated gold nanoclusters as specific bio-imaging probes for the detection of Hg(II) ions in in vivo and in vitro systems: discriminating between MDA-MB-231 and MCF10A cells

S. Chakraborty, A. Nandy, S. Ghosh, N. K. Das, S. Parveen, S. Datta and S. Mukherjee, Analyst, 2021, 146, 1455 DOI: 10.1039/D0AN02108C

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