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A proteolytic nanobiocatalyst with built-in disulphide reducing properties

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Abstract

We report a method to equip proteolytic nanobiocatalysts with intrinsic disulphide bond reducing properties. After immobilisation onto silica particles, selected protease enzymes are partially shielded in a nanometre-thick mercaptosilica layer acting not only as a protective system but also as a substrate reducing agent. The biocatalysts produced efficiently perform simultaneous disulphide bond reduction and protein digestion. Besides a significant simplification of the proteolysis process, this strategy allows for a drastic increase of the enzyme stability.

Graphical abstract: A proteolytic nanobiocatalyst with built-in disulphide reducing properties

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Supplementary files

Article information


Submitted
26 Nov 2020
Accepted
10 Dec 2020
First published
24 Dec 2020

This article is Open Access

RSC Adv., 2021,11, 810-816
Article type
Paper

A proteolytic nanobiocatalyst with built-in disulphide reducing properties

M. L. Briand, M. Bikaki, C. Puorger, P. F.-X. Corvini and P. Shahgaldian, RSC Adv., 2021, 11, 810
DOI: 10.1039/D0RA10013G

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