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Issue 13, 2021
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Metabolic labeling probes for interrogation of the host–pathogen interaction

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Abstract

Bacterial infections are still one of the leading causes of death worldwide; despite the near-ubiquitous availability of antibiotics. With antibiotic resistance on the rise, there is an urgent need for novel classes of antibiotic drugs. One particularly troublesome class of bacteria are those that have evolved highly efficacious mechanisms for surviving inside the host. These contribute to their virulence by immune evasion, and make them harder to treat with antibiotics due to their residence inside intracellular membrane-limited compartments. This has sparked the development of new chemical reporter molecules and bioorthogonal probes that can be metabolically incorporated into bacteria to provide insights into their activity status. In this review, we provide an overview of several classes of metabolic labeling probes capable of targeting either the peptidoglycan cell wall, the mycomembrane of mycobacteria and corynebacteria, or specific bacterial proteins. In addition, we highlight several important insights that have been made using these metabolic labeling probes.

Graphical abstract: Metabolic labeling probes for interrogation of the host–pathogen interaction

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Article information


Submitted
17 Dec 2020
Accepted
01 Mar 2021
First published
16 Mar 2021

This article is Open Access

Org. Biomol. Chem., 2021,19, 2856-2870
Article type
Review Article

Metabolic labeling probes for interrogation of the host–pathogen interaction

B. J. Ignacio, T. Bakkum, K. M. Bonger, N. I. Martin and S. I. van Kasteren, Org. Biomol. Chem., 2021, 19, 2856
DOI: 10.1039/D0OB02517H

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