Issue 6, 2021

Interrogating the transcriptome with metabolically incorporated ribonucleosides

Abstract

RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful strategy to facilitate molecular analysis of cellular RNA is the metabolic incorporation of chemical probes. In this review, we discuss current approaches for RNA metabolic labeling with modified ribonucleosides and their integration with Next-Generation Sequencing, mass spectrometry-based proteomics, and fluorescence microscopy in order to interrogate RNA behavior in its native context.

Graphical abstract: Interrogating the transcriptome with metabolically incorporated ribonucleosides

Article information

Article type
Review Article
Submitted
20 Aug 2021
Accepted
07 Oct 2021
First published
07 Oct 2021

Mol. Omics, 2021,17, 833-841

Interrogating the transcriptome with metabolically incorporated ribonucleosides

R. E. Kleiner, Mol. Omics, 2021, 17, 833 DOI: 10.1039/D1MO00334H

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