Issue 15, 2021

“One-to-three” droplet generation in digital microfluidics for parallel chemiluminescence immunoassays

Abstract

In digital microfluidics, droplet generation is a fundamental operation for quantitative liquid manipulation. The generation of well-defined micro-droplets on a chip with restricted device geometries has become a real obstacle for digital microfluidics platforms to be used in parallel for in vitro diagnostic applications. Here, we propose a “one-to-three” droplet splitting technique that is able to generate sub-microlitre droplets beyond the “well-known” geometry limit in electrowetting-on-dielectric digital microfluidics. Accordingly, we realized an on-chip magnetic bead chemiluminescence immunoassay for parallel detection with the “one-to-three” technique. With the help of the generated micro droplets, we were able to retain the magnetic beads by a significantly reduced magnetic force. We have shown the detection of five B-type natriuretic peptide analyte samples on a single chip for around 10 minutes. The correlation coefficient of the calibration curve was 0.9942, and the detection limit was lower than 5 pg mL−1.

Graphical abstract: “One-to-three” droplet generation in digital microfluidics for parallel chemiluminescence immunoassays

Supplementary files

Article information

Article type
Paper
Submitted
12 May 2021
Accepted
21 Jun 2021
First published
22 Jun 2021

Lab Chip, 2021,21, 2892-2900

“One-to-three” droplet generation in digital microfluidics for parallel chemiluminescence immunoassays

K. Jin, C. Hu, S. Hu, C. Hu, J. Li and H. Ma, Lab Chip, 2021, 21, 2892 DOI: 10.1039/D1LC00421B

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