Pinus koraiensis polyphenols: structural identification, in vitro antioxidant activity, immune function and inhibition of cancer cell proliferation

Abstract

Herein, polyphenols were extracted from Pinus koraiensis bark and characterized. Besides, the in vitro antioxidant activity, inhibition effect on cancer cells and the activity of the immune system were investigated. The results showed that the main component of Pinus koraiensis bark was 3,5,7,3′,5′-pentahydroxydihydroflavone. PKB polyphenols demonstrated a high antioxidant activity during in vitro investigation. In vivo immunological function studies on oxidatively injured mice revealed that Pinus koraiensis bark polyphenols effectively improved the survival status of irradiated mice. PKBP also increased the spleen and thymus index of mouse immunoregulatory organs. The results indicated that the phagocytic ability of mononuclear macrophages was increased. Comparing the cell distribution of the PKBP administered group and the model group, the PKBP-administered group reduced the cells arrested in the G₁ phase, while the number of cells increased in the S and G₂ phases. PKBP effectively protected the mouse immune system and reduced the immune suppression caused by radiation. These findings also confirmed that oxidative damaged cells induced by radiation could be repaired. PKBP had the highest inhibitory activity on colon cancer cells HT29, breast cancer cells MFC-7, gastric cancer cells BGC-823 and cervical cancer HeLa and HT29 cancer cells. PKB polyphenols could effectively induce the production of DNA-Ladder fragments and cause DNA damage in cancer cells. PKBP also blocked the cycle of cancer cells in the G₂ phase, stopped cell division and induced cancer cell apoptosis. Analysis of cell apoptosis by Annexin V-FTIC/PI double staining indicated that PKBP inhibited HT29 cancer cell proliferation.