Issue 76, 2021

Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region

Abstract

Full-length pharmaceutical antibodies, trastuzumab and rituximab, were chemically modified into Quenchbody, a fluorescent immunosensor, using a two-step reaction: (1) selective tyrosine residue modification of antibody complementarity determining regions (CDRs), and (2) introduction of fluorescent dye molecules by Cu-free click reaction. Without the need for genetic manipulation and time-consuming examination of protein expression conditions, the antibody–dye combination with good antigen response efficiency was examined in a simple two-hour operation.

Graphical abstract: Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region

Supplementary files

Article information

Article type
Communication
Submitted
18 Jun 2021
Accepted
26 Aug 2021
First published
26 Aug 2021

Chem. Commun., 2021,57, 9760-9763

Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region

S. Sato, M. Matsumura, H. Ueda and H. Nakamura, Chem. Commun., 2021, 57, 9760 DOI: 10.1039/D1CC03231C

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