Issue 15, 2021
From the journal:

Analyst

A fluorescent probe for STED microscopy to study NIP-specific B cells†

Selda Kabatas Glowacki,‡ab   Maria Angela Gomes de Castro, ORCID logo ‡a   Ka Man Yip,c   Ommolbanin Asadpour,ab   Matthias Münchhalfen,d   Niklas Engels*d  and  Felipe Opazo ORCID logo *ab  

* Corresponding authors

a Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany

b Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, Göttingen, Germany

c Department of Structural Dynamics, MPI for Biophysical Chemistry, Göttingen, Germany

d Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, Göttingen, Germany
E-mail: fopazo@gwdg.de, nengels@gwdg.de

Abstract

We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.

Graphical abstract: A fluorescent probe for STED microscopy to study NIP-specific B cells

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Article information

DOI
https://doi.org/10.1039/D1AN00601K
Article type
Communication
Submitted
09 Apr 2021
Accepted
23 Jun 2021
First published
30 Jun 2021
This article is Open Access
Creative Commons BY license

Analyst, 2021,146, 4744-4747

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A fluorescent probe for STED microscopy to study NIP-specific B cells

S. K. Glowacki, M. A. Gomes de Castro, K. M. Yip, O. Asadpour, M. Münchhalfen, N. Engels and F. Opazo, Analyst, 2021, 146, 4744 DOI: 10.1039/D1AN00601K

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