Amino acid modified gadofullerene protects against insulin resistance induced by oxidative stress in 3T3-L1 adipocytes
Reactive oxygen species (ROS) have been involved in the development of insulin resistance; thus, antioxidant therapy is a promising strategy in the treatment of insulin resistance related diseases. In this study, we synthesized a high-efficiency ROS scavenger, amino acid derivative of Gd@C82 (GF-Ala) and evaluated its effects on ROS-mediated insulin resistance in 3T3-L1 adipocytes. Exposure of adipocytes to H2O2 (generated by glucose oxidase) resulted in the elevated level of ROS, reduced content of GSH and increase of JNK activation, concomitantly with the decrease in insulin-stimulated PI3K, Akt and p70S6K activation and cellular glucose uptake. Pre-treatment of cells with GF-Ala suppressed H2O2-induced abnormity in cellular redox state and insulin signal pathway, attenuated insulin resistance in a dose-dependent manner. Together, gadofullerene has a potential therapeutic prospect in prevention and treatment of insulin resistance related diseases.