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Issue 30, 2020
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Single-molecule DNA origami aptasensors for real-time biomarker detection

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Abstract

Here we report the use of DNA nanostructures as platforms to monitor the inherent conformational changes of aptamers upon analyte binding, with single-molecule resolution and real-time capability. An aptasensor designed to sense cortisol was found to suffer from instability in solution, but this was reconciled via a rational design of a single-molecule sensing platform. In this regard, DNA origami was employed to immobilise individual aptasensors on a glass surface and to ensure adequate interaction with their environment, for single-molecule analysis. The strategy presented here can be applied to any aptamer obtained by the destabilisation of a duplex in a SELEX process, and hence employed in the rational design of single-molecule biosensors.

Graphical abstract: Single-molecule DNA origami aptasensors for real-time biomarker detection

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Supplementary files

Article information


Submitted
19 May 2020
Accepted
17 Jul 2020
First published
17 Jul 2020

This article is Open Access

J. Mater. Chem. B, 2020,8, 6352-6356
Article type
Communication

Single-molecule DNA origami aptasensors for real-time biomarker detection

K. Cervantes-Salguero, M. Freeley, J. L. Chávez and M. Palma, J. Mater. Chem. B, 2020, 8, 6352
DOI: 10.1039/D0TB01291B

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