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Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance

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Abstract

In tissue engineering, biological, physical, and chemical inputs have to be combined to properly mimic cellular environments and successfully build artificial tissues which can be designed to fulfill different biomedical needs such as the shortage of organ donors or the development of in vitro disease models for drug testing. Inclusion body-like protein nanoparticles (pNPs) can simultaneously provide such physical and biochemical stimuli to cells when attached to surfaces. However, this attachment has only been made by physisorption. To provide a stable anchoring, a covalent binding of lactic acid bacteria (LAB) produced pNPs, which lack the innate pyrogenic impurities of Gram-negative bacteria like Escherichia coli, is presented. The reported micropatterns feature a robust nanoscale topography with an unprecedented mechanical stability. In addition, they are denser and more capable of influencing cell morphology and orientation. The increased stability and the absence of pyrogenic impurities represent a step forward towards the translation of this material to a clinical setting.

Graphical abstract: Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance

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Supplementary files

Article information


Submitted
13 Mar 2020
Accepted
23 Apr 2020
First published
29 Apr 2020

This article is Open Access

J. Mater. Chem. B, 2020, Advance Article
Article type
Paper

Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance

M. Martínez-Miguel, A. R. Kyvik, L. M. Ernst, A. Martínez-Moreno, O. Cano-Garrido, E. Garcia-Fruitós, E. Vazquez, N. Ventosa, J. Guasch, J. Veciana, A. Villaverde and I. Ratera, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/D0TB00702A

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