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A label-free multifunctional nanosensor based on N-doped carbon nanodots for vitamin B12 and Co2+ detection, and bioimaging in living cells and zebrafish

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Abstract

Multifunctional N-doped carbon nanodots (N-CNDs) with a fluorescence (FL) quantum yield (QY) of 13.6% have been synthesized via a facile one-step hydrothermal process using Artemisia annua and 1,2-ethylenediamine as precursors. As-prepared N-CNDs showed excellent FL properties and were developed as a multifunctional sensing platform for vitamin B12 (VB12) and Co2+ determination, and bioimaging in living cells and zebrafish. The FL of N-CNDs is quenched efficiently in the presence of VB12 on the basis of the inner filter effect (IFE) or Co2+ by static quenching, respectively. EDTA as a masking agent enables Co2+ to be effectively eliminated and N-CNDs were used to selectively detect VB12 in the presence of both VB12 and Co2+. The present FL nanosensor can detect VB12 and Co2+ in the linear ranges of 0.5–35 μM and 2.5–25 μM with the corresponding detection limits of 47.4 nM and 230.5 nM, respectively. The study proved that the determination of Co2+ was based on the static quenching to form a complex between the amino group of N-CNDs and Co2+. Inspired by these outstanding properties, practical applications of this nanosensor for the detection of VB12 in actual samples (human serum, egg yolk, VB12 tablets and VB12 injection) and Co2+ in water samples were further verified with satisfactory results. The as-constructed N-CNDs have negligible toxicity and good biocompatibility, which facilitates utilization of N-CNDs in bioimaging of A549 cells and zebrafish, and sensing VB12 in living cells.

Graphical abstract: A label-free multifunctional nanosensor based on N-doped carbon nanodots for vitamin B12 and Co2+ detection, and bioimaging in living cells and zebrafish

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Supplementary files

Article information


Submitted
18 Feb 2020
Accepted
20 Apr 2020
First published
22 Apr 2020

J. Mater. Chem. B, 2020, Advance Article
Article type
Paper

A label-free multifunctional nanosensor based on N-doped carbon nanodots for vitamin B12 and Co2+ detection, and bioimaging in living cells and zebrafish

F. Du, Z. Cheng, M. Kremer, Y. Liu, X. Wang, S. Shuang and C. Dong, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/D0TB00443J

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