A novel mitochondria-targeted ratiometric fluorescent probe for endogenous sulfur dioxide derivatives as a cancer-detecting tool
A new mitochondria-targeted fluorescence probe RBC, constructed by a coumarin moiety which was selected as the donor and a benzothiazole derivative as the acceptor, for SO2 derivatives (HSO3-/ SO32-) was presented. The probe designed on a new FRET platform showed high selectivity and low detection limit. Importantly, the probe could respond to HSO3-/ SO32- within 35s. Furthermore, the probe could target mitochondria and was successfully used for fluorescence imaging of endogenous bisulfite in HepG2 with low cytotoxicity, which had a significantly assist to cancer diagnosis.