Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 20, 2020
Previous Article Next Article

Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation

Author affiliations

Abstract

Non-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positive ammonium groups and a tridecafullerene appended with 120 positive charges has been performed. The delivery of a plasmid containing the green fluorescent protein (EGFP) gene into HEK293 (Human Embryonic Kidney) cells resulting in effective gene expression has demonstrated the efficacy of these compounds to form polyplexes with DNA. Particularly, giant tridecafullerene macromolecules have shown higher efficiency in the complexation and transfection of DNA. Thus, they can be considered as promising non-viral vectors for transfection purposes.

Graphical abstract: Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation

Back to tab navigation

Supplementary files

Article information


Submitted
13 Jan 2020
Accepted
15 Apr 2020
First published
16 Apr 2020

J. Mater. Chem. B, 2020,8, 4505-4515
Article type
Paper

Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation

B. M. Illescas, A. Pérez-Sánchez, A. Mallo, Á. Martín-Domenech, I. Rodríguez-Crespo and N. Martín, J. Mater. Chem. B, 2020, 8, 4505
DOI: 10.1039/D0TB00113A

Social activity

Search articles by author

Spotlight

Advertisements