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Issue 7, 2020
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A FRET-based supramolecular nanoprobe with switch on red fluorescence to detect SO2 derivatives in living cells

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Abstract

SO2, an important gasotransmitter, plays significant roles in human physiological activities. The reported probes for the detection of SO2 derivatives are mostly based on the quenching or blue-shift of fluorescence, leading to interference from the background signal. Herein, we develop a novel FRET-based nanoprobe that shows turn-on fluorescence at 615 nm in the presence of SO32−/HSO3. The nanoprobe consists of N,O,B-chelated dipyrromethene (BDP) as the energy donor, and hemicyanine derivatives of HEM-CO-Ph with a responsive site to SO32−/HSO3 as the energy acceptor. Initially, the fluorescence of BDP is quenched by HEM-CO-Ph due to the efficient FRET process. In the presence of sulfites, the nucleophilic addition interrupts the conjugation of HEM-CO-Ph and results in a 200 nm blue-shift of the absorption, leading to the suppression of the FRET process and recovery of the red fluorescence of BDP. The nanoprobe shows fast response to SO32−/HSO3 with high selectivity, great biocompatibility and cellular uptake. It is the first supramolecular nanoprobe for the detection of SO32−/HSO3 by using intermolecular energy transfer, ingeniously turning the nanoprobe from “OFF” state to “ON” state. The nanoprobe is applied to monitor exogenous and endogenous SO2 derivatives in the mitochondria of living cells.

Graphical abstract: A FRET-based supramolecular nanoprobe with switch on red fluorescence to detect SO2 derivatives in living cells

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Supplementary files

Article information


Submitted
10 Dec 2019
Accepted
21 Jan 2020
First published
24 Jan 2020

J. Mater. Chem. B, 2020,8, 1538-1544
Article type
Paper

A FRET-based supramolecular nanoprobe with switch on red fluorescence to detect SO2 derivatives in living cells

L. Jia, L. Niu, L. Wang, X. Wang and Q. Yang, J. Mater. Chem. B, 2020, 8, 1538
DOI: 10.1039/C9TB02797A

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