A Novel Cross-linked Nanoparticle With Aggregation-induced Emission Properties for Cancer Cells Imaging
Fluorescent probes have been widely used in bioimaging as an efficient and convenient analytical method. From the initial inorganic nanoparticles and small organic molecules to polymeric nanoparticles, scientific researchers have been trying to develop a probe with strong fluorescence and excellent biocompatibility. In this study, a a tetraphenylethylene derivative with AIE and hyaluronic acid modified by methacrylic anhydride was combined to prepare a novel nanoparticle (HA-Ac-Pha-C) as fluorescent probe by photochemical cross-linking reaction. The fluorescence intensity and size of nanoparticles were characterized by different techniques. It was confirmed that cross-linked nanoparticles not only showed stronger fluorescence, but also had better photostability while still maintained 85.9% of initial intensity after seven days. Moreover, cells and zebrafish imaging experiments also demonstrated that nanoparticles have specific fluorescent labeling for cancer cells and excellent biocompatibility in living organisms.