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A novel “on–off–on” fluorescence assay for the discriminative detection of Cu(ii) and l-cysteine based on red-emissive Si-CDs and cellular imaging applications

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Abstract

Copper ions (Cu2+) and L-cysteine (L-Cys) in the human body always play critical roles in various physiological processes, while abnormal Cu2+ and L-Cys concentrations in the biological system lead to many diseases. In this manuscript, Si-doped carbon dots (Si-CDs) with near-infrared fluorescence were designed for the detection of Cu2+ and L-Cys through the fluorescence “on–off–on” mode. The carbon dots exhibited not only excellent optical merits including good stability against photobleaching and high chemical stability, but also superior biological compatibility. Interestingly, due to the abundant amino groups distributed on the surface of Si-CDs, they could bind to copper ions to form cupric amine complexes and then quench the fluorescence of Si-CDs due to an electron transfer process. In addition, upon the addition of L-Cys, the FL intensity of Si-CDs could be effectively recovered accompanied with complexation between Cu2+ and the functional groups in L-Cys, due to which Cu2+ was removed from the surface of Si-CDs. Notably, as far as we know, these are the first red-emitting carbon dots for copper ion and L-Cys assays in water samples and human plasma samples. Furthermore, this strategy was successfully applied to the determination of Cu2+ and L-Cys in living systems, demonstrating great practicability in biomedical applications.

Graphical abstract: A novel “on–off–on” fluorescence assay for the discriminative detection of Cu(ii) and l-cysteine based on red-emissive Si-CDs and cellular imaging applications

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Supplementary files

Article information


Submitted
27 Nov 2019
Accepted
09 Dec 2019
First published
15 Dec 2019

J. Mater. Chem. B, 2020, Advance Article
Article type
Paper

A novel “on–off–on” fluorescence assay for the discriminative detection of Cu(II) and L-cysteine based on red-emissive Si-CDs and cellular imaging applications

M. Zan, C. Li, D. Zhu, L. Rao, Q. Meng, B. Chen, W. Xie, X. Qie, L. Li, X. Zeng, Y. Li, W. Dong and W. Liu, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB02681A

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