Issue 7, 2020

A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell

Abstract

A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification. A nitrogen doped ultra-thin carbon shell/gold nanoparticle (N-UHCS/AuNPs) composite was prepared and applied as an electrode supporting substrate to improve the enzyme load. The biocathode of the self-powered sensor is constructed through the step-by-step modification of N-UHCS/AuNPs and bilirubin oxidase (BOD) on carbon paper (CP). To fabricate the bioanode, SiO2 nanospheres@AuNPs–aptamer (SiO2@AuNPs–ssDNA) bioconjugates were prepared and modified on CP. When there is a target protein, the aptamer recognizes it and causes the SiO2@AuNPs–ssDNA bioconjugate to fall off the bioanode, resulting in a significant increase in the open circuit voltage (EOCV) of the sensing device. Under optimal conditions, the developed biosensor shows a wide linear range of 0.1–2000 ng mL−1 with a low detection limit of 21.5 pg mL−1 (S/N = 3). This work shows an effective assay for the sensitive detection of biomolecules by coupling EBFCs, DNA bioconjugates and the biosensing characteristics of smart nanostructures.

Graphical abstract: A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell

Article information

Article type
Paper
Submitted
15 Nov 2019
Accepted
10 Jan 2020
First published
11 Jan 2020

J. Mater. Chem. B, 2020,8, 1389-1395

A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell

F. Wang, Y. Wang, J. Xu and K. Huang, J. Mater. Chem. B, 2020, 8, 1389 DOI: 10.1039/C9TB02574J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements