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Modulating the properties of flow-assembled chitosan membranes in microfluidics with glutaraldehyde crosslinking

Abstract

Flow-assembled chitosan membranes are robust and semipermeable hydrogel structures formed in microfluidic devices that have been used for important applications such as gradient generation and studying cell-cell signaling. One challenge, however, remains unresolved. When a polydimethylsiloxane (PDMS) microchannel with the flow-assembled, deprotonated chitosan membrane (DCM) is treated with anti-adhesion agents such as Pluronic F-127 to prevent biomolecular and cellular adsorption on PDMS, the interaction between DCM and PDMS is compromised and the DCM easily delaminates. To address this challenge, DCMs in microfludics are crosslinked with glutaraldehyde to modulate its properties, and the altered properties of the glutaraldehyde treated chitosan membrane (GTCM) were investigated. First, GTCM’s acidic resistance was confirmed, it's mechanical robustness against hydrostatic pressure was significantly improved, and it remained intact on PDMS after Pluronic treatment. Second, crystallization in DCM and GTCM was investigated with quantitative polarized light microscopy (qPLM), which revealed that GTCM’s optical retardance and anisotropy were lower, implying less molecular alignment than DCM. Finally, membrane permeability was tested with FITC-labeled dextran transport experiments, which showed that the transport across GTCM was slightly higher than that across DCM. Overall, glutaraldehyde-crosslinked chitosan membrane has better acidic resistance, higher strength under Pluronic treatment, and less molecular microalignment, while its semi-permeability is retained. This study demonstrates how glutaraldehyde crosslinking can be used to modify and improve biopolymer membrane properties for broader applications such as an environment is acidic or when a Pluronic passivation is needed.

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Article information


Submitted
09 Nov 2019
Accepted
14 Feb 2020
First published
14 Feb 2020

J. Mater. Chem. B, 2020, Accepted Manuscript
Article type
Paper

Modulating the properties of flow-assembled chitosan membranes in microfluidics with glutaraldehyde crosslinking

P. Hu, C. Raub, J. S. Choy and X. Luo, J. Mater. Chem. B, 2020, Accepted Manuscript , DOI: 10.1039/C9TB02527H

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