Jump to main content
Jump to site search


Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

Author affiliations

Abstract

In this study, we have developed a rapid and cost-effective method employing platinum ion (Pt4+)-capped fluorescent carbon quantum dots (CQDs) coupled with loop-mediated isothermal amplification (LAMP) to detect dual MRSA genes. We synthesized nitrogen- and chlorine-co-doped fluorescent CQDs (CQDSPDs) from spermidine trihydrochloride via a simple one-step pyrolysis. The CQDSPDs capped with Pt4+ ions through the cooperative coordination of the amine and chlorine groups on the surface of CQDs facilitated the double-stranded DNA (dsDNA)-induced fluorescence quenching of CQDs, and enabled the construction of the CQDSPDs/Pt4+ probe for the detection of as few as 10 copies of the MRSA gene (mecA and femA). The sensitivity and specificity of the CQDSPDs/Pt4+ probe for MRSA detection in clinical specimens (n = 24) were 94% and 86%, respectively. Our results reveal that the CQDSPDs/Pt4+ probe has great potential for the diagnosis of antibiotic-resistant superbugs with high sensitivity, specificity, and agreement.

Graphical abstract: Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

Back to tab navigation

Supplementary files

Article information


Submitted
04 Nov 2019
Accepted
06 Dec 2019
First published
06 Dec 2019

J. Mater. Chem. B, 2020, Advance Article
Article type
Communication

Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

H. Li, J. Mao, C. Lien, C. Wang, J. Lai, R. P. Mandal, H. Chang, L. Chang, D. H. Ma and C. Huang, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB02468A

Social activity

Search articles by author

Spotlight

Advertisements