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The design and characterization of a hypersensitive glucose sensor: two enzymes co-fixed on a copper phosphate skeleton

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Abstract

In this study, we first reported a new glucose sensor for the preparation of a glucose oxidase (GOx)-conjugated novel artificial peroxidase mimic deuterohemin peptide (Dh-Ala-His-Thr-Val-Glu-Lys, DhHP-6) (GOx&DhHP-6–Cu3(PO4)2) in order to determine the glucose concentration. The same catalytic environment (pH 7.0, 35 °C) between glucose oxidase (GOx) and DhHP-6 ensured their utilization in catalytic cascade reactions. DhHP-6 and GOx were co-fixed on a copper phosphate skeleton, which ensured resistance toward harsh environments and improved storage stability. Meanwhile, the sensor reduced the diffusion of hydrogen peroxide (H2O2) in solutions by immobilization. GOx&DhHP-6–Cu3(PO4)2 possessed a wide linear range of 5–2000 μM, and a limit of detection of 0.5 μM. Based on the above-mentioned results, a new method to rapidly detect glucose concentrations was successfully developed using GOx&DhHP-6–Cu3(PO4)2 nanoflowers with high sensitivity and an easily applied methodology.

Graphical abstract: The design and characterization of a hypersensitive glucose sensor: two enzymes co-fixed on a copper phosphate skeleton

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Publication details

The article was received on 17 Oct 2019, accepted on 14 Nov 2019 and first published on 25 Nov 2019


Article type: Paper
DOI: 10.1039/C9TB02294E
J. Mater. Chem. B, 2020, Advance Article

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    The design and characterization of a hypersensitive glucose sensor: two enzymes co-fixed on a copper phosphate skeleton

    Y. Yuan, Z. Fu, K. Wang, Z. Zhao, H. Li, Z. Wang and L. Wang, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB02294E

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