Jump to main content
Jump to site search


Characterization and application of fluidic properties of trinucleotide repeat sequences by wax-on-plastic microfluidics

Author affiliations

Abstract

Trinucleotide repeat (TNR) sequences introduce sequence-directed flexibility in the genomic makeup of all living species leading to unique non-canonical structure formation. In humans, the expansions of TNR sequences are responsible for almost 24 neurodegenerative and neuromuscular diseases because their unique structures disrupt cell functions. The biophysical studies of these sequences affect their electrophoretic mobility and spectroscopic signatures. Here, we demonstrate a novel strategy to characterize and discriminate the TNR sequences by monitoring their capillary flow in the absence of an external driving force using wax-on-plastic microchannels. The wax-on-plastic microfluidic system translates the sequence-directed flexibility of TNR into differential flow dynamics. Several variables were used to characterize sequences including concentration, single- vs. double-stranded samples, type of repeat sequence, length of the repeat sequence, presence of mismatches in duplex, and presence of metal ion. All these variables were found to influence the flow velocities of TNR sequences as these factors directly affect the structural flexibility of TNR at the molecular level. An overall trend was observed as the higher flexibility in the TNR structure leads to lower capillary flow. After testing samples derived from relevant cells harboring expanded TNR sequences, it is concluded that this approach may transform into a reagent-free and pump-free biosensing platform to detect microsatellite expansion diseases.

Graphical abstract: Characterization and application of fluidic properties of trinucleotide repeat sequences by wax-on-plastic microfluidics

Back to tab navigation

Supplementary files

Article information


Submitted
08 Oct 2019
Accepted
18 Dec 2019
First published
19 Dec 2019

J. Mater. Chem. B, 2020, Advance Article
Article type
Paper

Characterization and application of fluidic properties of trinucleotide repeat sequences by wax-on-plastic microfluidics

A. Z. Qamar, N. Asefifeyzabadi, M. Taki, S. Naphade, L. M. Ellerby and M. H. Shamsi, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB02208B

Social activity

Search articles by author

Spotlight

Advertisements