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A colorimetric and electrochemical dual-mode biosensor for thrombin using a magnetic separation technique

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Abstract

In general, protein detection relies primarily on enzyme-linked immunosorbent assays. Here, we constructed a colorimetric and electrochemical dual-mode biosensor for thrombin detection based on the mechanism of aptamer recognition. Magnetic nanobeads (MBs) were used as carriers for separation and enrichment to quickly capture thrombin (TB) in the complex matrix. Also, the combination of MBs and the magnetic electrode array (MEA) effectively avoided the poisoning of the electrode by biological samples. Furthermore, hybridization chain reaction (HCR) was indirectly used to achieve amplification of TB. A large number of horseradish peroxidases (HRPs) were coupled with the amplified long nucleic acid fragments. Based on the color and current response of the substrate TMB catalyzed by HRP, a dual-mode detection system for thrombin was established to ensure the accuracy of the test results. The method had a minimum resolution of 10 nM to the naked eye and an electrochemical detection limit as low as 0.35 nM. In addition, the sensor provided good anti-interference ability in a complex matrix and showed great potential to detect TB in complex samples.

Graphical abstract: A colorimetric and electrochemical dual-mode biosensor for thrombin using a magnetic separation technique

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Article information


Submitted
04 Oct 2019
Accepted
01 Nov 2019
First published
02 Nov 2019

J. Mater. Chem. B, 2020, Advance Article
Article type
Paper

A colorimetric and electrochemical dual-mode biosensor for thrombin using a magnetic separation technique

W. Guo, X. Yang, Z. Wu and Z. Zhang, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB02170A

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