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In situ derivatization of Au nanoclusters via aurophilic interactions of a triphenylphosphine gold(i) salt with neurotransmitters and their rapid MALDI-TOF-MS detection in mice brain tissue extracts

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Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has attracted much attention for the detection of small molecules such as neurotransmitters due to its softness, high sensitivity, extensive compatibility and diverse mass analyzers. However, it has been really a difficult challenge to develop a highly specific organic compound as a matrix for the rapid, sensitive and selective detection of neurotransmitters. Herein, we report tris(triphenylphosphine)gold oxonium tetrafluoroborate ([Ph3PAu]3O+BF4) for the first time as an efficient matrix for the rapid and simultaneous MALDI-MS detection of neurotransmitters. [Ph3PAu]3O+BF4 facilitates the in situ derivatization of gold nanoclusters (Au NCLs) during the interaction with neurotransmitters, which increases their ionization energy by absorbing more ultra-violet (UV) radiation during MALDI-TOF-MS detection. The results show that this [Ph3PAu]3O+BF4 matrix can exhibit a 10-fold faster response time compared to previously reported pyrylium matrices. In addition, [Ph3PAu]3O+BF4 can also provide the simultaneous derivatization of various neurotransmitters, including dopamine (DA), noradrenaline (NAd), serotonin (5-HT), γ-aminobutyric acid (GABA), histamine (H) and tyramine (TY), in mice brain tissue extracts, which can be detected in the MALDI-TOF-MS spectra.

Graphical abstract: In situ derivatization of Au nanoclusters via aurophilic interactions of a triphenylphosphine gold(i) salt with neurotransmitters and their rapid MALDI-TOF-MS detection in mice brain tissue extracts

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Publication details

The article was received on 22 Aug 2019, accepted on 30 Oct 2019 and first published on 04 Nov 2019


Article type: Paper
DOI: 10.1039/C9TB01800J
J. Mater. Chem. B, 2020, Advance Article

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    In situ derivatization of Au nanoclusters via aurophilic interactions of a triphenylphosphine gold(I) salt with neurotransmitters and their rapid MALDI-TOF-MS detection in mice brain tissue extracts

    S. Palanisamy, S. Huang, H. Zhao, D. Zhu and X. Zhang, J. Mater. Chem. B, 2020, Advance Article , DOI: 10.1039/C9TB01800J

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