Membrane reinforcement in Giant Hybrid Polymer Lipid Vesicle achieved by controlling the polymer architecture
Physical properties of membranes of hybrid polymer lipid vesicles are so far relatively unknown. Since their discovery a decade ago, many studies have aimed to show their high potential in many fields of application, but so far, few systematic studies have been carried out to decipher the relationship between the molecular characteristic of the components (molar mass, chemical nature, architecture of the copolymer), the membrane structuration and its properties. In this work, we study the association of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and poly(dimethylsiloxane)-b-poly(ethylene oxide) (PDMS-b-PEO) diblock copolymers of different molar masses in giant hybrid vesicle and establish a complete phase diagram of the membrane structuration. We also measure the mechanical properties of the GHUV through micropipette aspiration at different lipid/polymer compositions. Thanks to a previous work using triblock PEO-b-PDMS-b-PEO copolymers, we were able to reveal the effect of the architecture of block copolymer on membrane structuration and properties. Besides, the association of diblock copolymers PDMS-b-PEO with POPC lead to the formation of hybrid vesicle with unprecedented membrane toughness.