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Holographic immunoassays: direct detection of antibodies binding to colloidal spheres

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Abstract

The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies’ binding rates and can be inverted to obtain the concentration of antibodies in solution. Holographic molecular binding assays therefore can be used to perform fast quantitative immunoassays that are complementary to conventional serological tests.

Graphical abstract: Holographic immunoassays: direct detection of antibodies binding to colloidal spheres

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Supplementary files

Article information


Submitted
24 Jul 2020
Accepted
05 Oct 2020
First published
08 Oct 2020

Soft Matter, 2020, Advance Article
Article type
Paper

Holographic immunoassays: direct detection of antibodies binding to colloidal spheres

K. Snyder, R. Quddus, A. D. Hollingsworth, K. Kirshenbaum and D. G. Grier, Soft Matter, 2020, Advance Article , DOI: 10.1039/D0SM01351J

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