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Issue 26, 2020
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Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

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Abstract

The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.

Graphical abstract: Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

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Supplementary files

Article information


Submitted
13 May 2020
Accepted
02 Jun 2020
First published
08 Jun 2020

Soft Matter, 2020,16, 6063-6071
Article type
Paper

Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

H. C. Moon, S. Han, J. Borges, T. Pesqueira, H. Choi, S. Y. Han, H. Cho, J. H. Park, J. F. Mano and I. S. Choi, Soft Matter, 2020, 16, 6063
DOI: 10.1039/D0SM00876A

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