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Issue 24, 2020
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Single-cell biophysical study reveals deformability and internal ordering relationship in T cells

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Abstract

Deformability and internal ordering are key features related to cell function, particularly critical for cells that routinely undergo large deformations, like T cells during extravasation and migration. In the measurement of cell deformability, a considerable variability is typically obtained, masking the identification of possible interrelationships between deformability, internal ordering and cell function. We report the development of a single-cell methodology that combines measurements of living-cell deformability, using micropipette aspiration, and three-dimensional confocal analysis of the nucleus and cytoskeleton. We show that this single-cell approach can serve as a powerful tool to identify appropriate parameters that characterize deformability within a population of cells, not readably discernable in population-averaged data. By applying this single-cell methodology to mouse CD4+ T cells, our results demonstrate that the relative size of the nucleus, better than other geometrical or cytoskeletal features, effectively determines the overall deformability of the cells within the population.

Graphical abstract: Single-cell biophysical study reveals deformability and internal ordering relationship in T cells

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Supplementary files

Article information


Submitted
11 Apr 2020
Accepted
02 Jun 2020
First published
03 Jun 2020

Soft Matter, 2020,16, 5669-5678
Article type
Paper

Single-cell biophysical study reveals deformability and internal ordering relationship in T cells

B. González-Bermúdez, H. Kobayashi, Á. Navarrete, C. Nyblad, M. González-Sánchez, M. de la Fuente, G. Fuentes, G. V. Guinea, C. García and G. R. Plaza, Soft Matter, 2020, 16, 5669
DOI: 10.1039/D0SM00648C

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