Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Finger-like membrane protrusions are favored by heterogeneities in the actin network

Author affiliations

Abstract

Finger-like protrusions in cells are mostly generated by an active actin cytoskeleton pushing against the cell membrane. Conventional filopodia, localized at the leading edge of the cells, are long and thin protrusions composed of parallel actin filaments that emanate from a branched actin network. In contrast, dendritic filopodia, precursors of dendritic spines in neurons, are entirely filled in with a branched actin network. Here, we investigate in vitro how the dynamics of branched actin structures, polymerized at a membrane surface, trigger the formation of both protrusion types. Using supported bilayers and liposomes, we show that a decrease in the amount of activation sites at the membrane surface leads to the appearance of heterogeneities in the actin network coverage. Such heterogeneities promote the formation of membrane protrusions, and the size of heterogeneity patches matches the one of the protrusion base. Protrusion shape, cylindrical or conical, directly correlates with the absence or the presence of actin branches, respectively.

Graphical abstract: Finger-like membrane protrusions are favored by heterogeneities in the actin network

Back to tab navigation

Supplementary files

Article information


Submitted
12 Dec 2019
Accepted
12 May 2020
First published
13 May 2020

Soft Matter, 2020, Advance Article
Article type
Paper

Finger-like membrane protrusions are favored by heterogeneities in the actin network

S. Gat, C. Simon, C. Campillo, A. Bernheim-Groswasser and C. Sykes, Soft Matter, 2020, Advance Article , DOI: 10.1039/C9SM02444A

Social activity

Search articles by author

Spotlight

Advertisements