Jump to main content
Jump to site search


Lipid-anchor display on peptoid nanosheets via co-assembly for multivalent pathogen recognition

Author affiliations

Abstract

Biological systems have evolved sophisticated molecular assemblies capable of exquisite molecular recognition across length scales ranging from angstroms to microns. For instance, the self-organization of glycolipids and glycoproteins on cell membranes allows for molecular recognition of a diversity of ligands ranging from small molecules and proteins to viruses and whole cells. A distinguishing feature of these 2D surfaces is they achieve exceptional binding selectivity and avidity by exploiting multivalent binding interactions. Here we develop a 2D ligand display platform based on peptoid nanosheets that mimics the structure and function of the cell membrane. A variety of small-molecule lipid-conjugates were co-assembled with the peptoid chains to create a diversity of functionalized nanosheet bilayers with varying display densities. The functional heads of the lipids were shown to be surface-exposed, and the carbon tails immobilized into the hydrophobic interior. We demonstrate that saccharide-functionalized nanosheets (e.g., made from globotriaosylsphingosine or 1,2-dipalmitoyl-sn-glycero-3-phospho((ethyl-1′,2′,3′-triazole)triethyleneglycolmannose)) can have very diverse binding properties, exhibiting specific binding to multivalent proteins as well as to intact bacterial cells. Analysis of sugar display densities revealed that Shiga toxin 1 subunit B (a pentameric protein) and FimH-expressing Escherichia coli (E. coli) bind through the cooperative binding behavior of multiple carbohydrates. The ability to readily incorporate and display a wide variety of lipidated cargo on the surface of peptoid nanosheets makes this a convenient route to soluble, cell-surface mimetic materials. These materials hold great promise for drug screening, biosensing, bioremediation, and as a means to combat pathogens by direct physical binding through a well-defined, multivalent 2D material.

Graphical abstract: Lipid-anchor display on peptoid nanosheets via co-assembly for multivalent pathogen recognition

Back to tab navigation

Supplementary files

Article information


Submitted
22 Sep 2019
Accepted
09 Dec 2019
First published
19 Dec 2019

Soft Matter, 2020, Advance Article
Article type
Paper

Lipid-anchor display on peptoid nanosheets via co-assembly for multivalent pathogen recognition

J. H. Kim, E. M. Grzincic, L. Yun, R. K. Spencer, M. A. Kline and R. N. Zuckermann, Soft Matter, 2020, Advance Article , DOI: 10.1039/C9SM01908A

Social activity

Search articles by author

Spotlight

Advertisements