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Particle diffusion in extracellular hydrogels

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Abstract

Hyaluronic acid is an abundant polyelectrolyte in the human body that forms extracellular hydrogels in connective tissues. It is essential for regulating tissue biomechanics and cell–cell communication, yet hyaluronan overexpression is associated with pathological situations such as cancer and multiple sclerosis. Due to its enormous molecular weight (in the range of millions of Daltons), accumulation of hyaluronan hinders transport of macromolecules including nutrients and growth factors through tissues and also hampers drug delivery. However, the exact contribution of hyaluronan to tissue penetrability is poorly understood due to the complex structure and molecular composition of tissues. Here we reconstitute biomimetic hyaluronan gels and systematically investigate the effects of gel composition and crosslinking on the diffusion of microscopic tracer particles. We combine ensemble-averaged measurements via differential dynamic microscopy with single-particle tracking. We show that the particle diffusivity depends on the particle size relative to the network pore size and also on the stress relaxation dynamics of the network. We furthermore show that addition of collagen, the other major biopolymer in tissues, causes the emergence of caged particle dynamics. Our findings are useful for understanding macromolecular transport in tissues and for designing biomimetic extracellular matrix hydrogels for drug delivery and tissue regeneration.

Graphical abstract: Particle diffusion in extracellular hydrogels

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Article information


Submitted
11 Sep 2019
Accepted
27 Dec 2019
First published
08 Jan 2020

This article is Open Access

Soft Matter, 2020, Advance Article
Article type
Paper

Particle diffusion in extracellular hydrogels

F. Burla, T. Sentjabrskaja, G. Pletikapic, J. van Beugen and G. H. Koenderink, Soft Matter, 2020, Advance Article , DOI: 10.1039/C9SM01837A

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