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Identification of a nanomolar affinity α-synuclein fibril imaging probe by ultra-high throughput in silico screening

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Abstract

Small molecules that bind with high affinity and specificity to fibrils of the α-synuclein (αS) protein have the potential to serve as positron emission tomography (PET) imaging probes to aid in the diagnosis of Parkinson's disease and related synucleinopathies. To identify such molecules, we employed an ultra-high throughput in silico screening strategy using idealized pseudo-ligands termed exemplars to identify compounds for experimental binding studies. For the top hit from this screen, we used photo-crosslinking to confirm its binding site and studied the structure–activity relationship of its analogs to develop multiple molecules with nanomolar affinity for αS fibrils and moderate specificity for αS over Aβ fibrils. Lastly, we demonstrated the potential of the lead analog as an imaging probe by measuring binding to αS-enriched homogenates from mouse brain tissue using a radiolabeled analog of the identified molecule. This study demonstrates the validity of our powerful new approach to the discovery of PET probes for challenging molecular targets.

Graphical abstract: Identification of a nanomolar affinity α-synuclein fibril imaging probe by ultra-high throughput in silico screening

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Supplementary files

Article information


Submitted
15 Apr 2020
Accepted
31 Aug 2020
First published
10 Sep 2020

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2020, Advance Article
Article type
Edge Article

Identification of a nanomolar affinity α-synuclein fibril imaging probe by ultra-high throughput in silico screening

J. J. Ferrie, Z. Lengyel-Zhand, B. Janssen, M. G. Lougee, S. Giannakoulias, C. Hsieh, V. V. Pagar, C. Weng, H. Xu, T. J. A. Graham, V. M.-Y. Lee, R. H. Mach and E. J. Petersson, Chem. Sci., 2020, Advance Article , DOI: 10.1039/D0SC02159H

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