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In vivo monitoring tissue regeneration by a ratiometric lysosomal AIE probe

Abstract

Tissue regeneration is a crucial self-renewal capability involving many complex biological processes. Although transgenic techniques and fluorescence immunohistochemical staining have promoted our understanding of tissue regeneration, simultaneous quantification and visualization of tissue regeneration processes is not easy to achieve. Herein, we developed a simple and quantitative method for the real-time and non-invasive observation of the process of tissue regeneration. The synthesized ratiometric aggregation-induced-emission (AIE) probe exhibits high selectivity and reversibility for pH responses, good ability of mapping lysosomal pH both in vitro and in vivo, good biocompatibility and excellent photostability. The caudal fin regeneration of a fish model (medaka larvae) was monitored by tracking the lysosomal pH change. It was found that the mean lysosomal pH is reduced during 24–48 hpa to promote the autophagic activity for cell debris degradation. Our research can quantify the changes in mean lysosomal pH and also exhibit its distribution during the caudal fin regeneration. We believe that the AIE-active lysosomal pH probe can also be potentially used for long-term tracking of various lysosome-involved biological processes, such as tracking the stress responses of tissue, tracking the inflammatory responses, and so on.

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Supplementary files

Article information


Submitted
09 Dec 2019
Accepted
07 Feb 2020
First published
11 Feb 2020

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2020, Accepted Manuscript
Article type
Edge Article

In vivo monitoring tissue regeneration by a ratiometric lysosomal AIE probe

X. Shi, N. YAN, G. Niu, S. H. P. Sung, Z. Liu, J. Liu, R. T. K. Kwok, J. W. Y. Lam, W. Wang, H. H-Y. Sung, I. D. Williams and B. Z. Tang, Chem. Sci., 2020, Accepted Manuscript , DOI: 10.1039/C9SC06226B

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