Jump to main content
Jump to site search


Protein Encapsulation: A New Approach for Improving the Capability of Small-Molecule Fluorogenic Probes

Abstract

Herein, we report a protein-based hybridization strategy that exploits the host-guest chemistry of HSA (human serum albumin) to solubilize the otherwise cell impermeable ONOO– fluorescent probe Pinkment-OAc. Formation of a HSA/Pinkment-OAc supramolecular hybrid was confirmed by SAXS and solution-state analyses. This HSA/Pinkment-OAc hybrid provided an enhanced fluorescence response towards ONOO– versus Pinkment-OAc alone, as determined by in vitro experiments. The HSA/Pinkment-OAc hybrid was also evaluated in the RAW264.7 macrophage and HeLa cancer cell lines, which displayed an enhanced cell permeability enabling the detection of SIN-1 generated ONOO– and the in vivo imaging of acute inflammation in LPS-treated mice. A remarkable 5.4- and 2.7-fold increased response was seen relative to Pinkment-OAc alone at the cellular level and in vivo, respectively. We anticipate that these HSA/fluorescent probe hybrids will soon become ubiquitous and routinely applied to overcome solubility issues associated with hydrophobic fluorescent imaging agents designed to detect disease related biomarkers

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Aug 2019, accepted on 26 Nov 2019 and first published on 27 Nov 2019


Article type: Edge Article
DOI: 10.1039/C9SC03961A
Chem. Sci., 2020, Accepted Manuscript
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

  •   Request permissions

    Protein Encapsulation: A New Approach for Improving the Capability of Small-Molecule Fluorogenic Probes

    H. Han, A. C. Sedgwick, Y. Shang, N. Li, T. Liu, B. Li, K. Yu, Y. Zang, J. T. Brewster II, M. L. Odyniec , M. Weber, S. Bull, J. Li, J. L. Sessler, T. D. James, X. He and H. Tian, Chem. Sci., 2020, Accepted Manuscript , DOI: 10.1039/C9SC03961A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements