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Issue 50, 2020
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High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

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Abstract

Plasmonic gold nanorods (AuNRs) have been widely applied as optical orientation probes in many biophysical studies. However, characterizing the various three-dimensional (3D) orientations of AuNRs in the same focal plane of the objective lens is a challenging task. To overcome this challenge, we fabricated single AuNRs (10 nm × 30 nm) coated with either an elliptical or spherical mesoporous silica shell (AuNRs@mSiO2). Unlike bare AuNRs and elliptical AuNRs@mSiO2, spherical AuNRs@mSiO2 contained randomly oriented AuNR cores in 3D space, which could be observed on the same focal plane within a single frame by differential interference contrast (DIC) microscopy. The spherical AuNRs@mSiO2 thus achieved high-throughput detection. The proposed approach can overcome the limitations of the current gel-matrix method, which requires vertical scanning of the embedded AuNRs to capture different focal planes.

Graphical abstract: High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

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Supplementary files

Article information


Submitted
28 May 2020
Accepted
02 Aug 2020
First published
12 Aug 2020

This article is Open Access

RSC Adv., 2020,10, 29868-29872
Article type
Paper

High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

G. W. Kim, S. Yoon, J. H. Lee and J. W. Ha, RSC Adv., 2020, 10, 29868
DOI: 10.1039/D0RA04704J

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