Issue 40, 2020

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

Graphical abstract: A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

Supplementary files

Article information

Article type
Paper
Submitted
14 Mar 2020
Accepted
16 Jun 2020
First published
23 Jun 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 23931-23935

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

C. C. Popescu, M. C. Stoian, L. Cucos, A. G. Coman, A. Radoi, A. Paun, N. D. Hădade, A. Gautier, C. Popescu and M. Matache, RSC Adv., 2020, 10, 23931 DOI: 10.1039/D0RA02382E

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