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Issue 40, 2020
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A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

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Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

Graphical abstract: A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

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Supplementary files

Article information


Submitted
14 Mar 2020
Accepted
16 Jun 2020
First published
23 Jun 2020

This article is Open Access

RSC Adv., 2020,10, 23931-23935
Article type
Paper

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

C. C. Popescu, M. C. Stoian, L. Cucos, A. G. Coman, A. Radoi, A. Paun, N. D. Hădade, A. Gautier, C. Popescu and M. Matache, RSC Adv., 2020, 10, 23931
DOI: 10.1039/D0RA02382E

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    [Original citation] - Published by The Royal Society of Chemistry.

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