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Issue 14, 2020
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Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation

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Abstract

Quantum dots (QDs) are promising nanomaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD surface functionalisation in biological applications.

Graphical abstract: Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation

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Supplementary files

Article information


Submitted
16 Jan 2020
Accepted
17 Feb 2020
First published
26 Feb 2020

This article is Open Access

RSC Adv., 2020,10, 8218-8223
Article type
Paper

Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation

T. Suwatthanarak, M. Tanaka, T. Minamide, A. J. Harvie, A. Tamang, K. Critchley, S. D. Evans and M. Okochi, RSC Adv., 2020, 10, 8218
DOI: 10.1039/D0RA00460J

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    [Original citation] - Published by The Royal Society of Chemistry.

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