Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

Abstract

Nanoparticle-assisted deep-tissue fluorescence imaging is of great significance to bioimaging and disease diagnosis. Through the combination of the electron donor triphenylamine and electron acceptor cyan, the compound 1,1-dicyano-2-phenyl-2-(4-diphenylamino)phenylethylene (DCPE-TPA, 1) was synthesized and exhibited an aggregation-induced emission (AIE) effect. Compound 1 can form three kinds of aggregation states: orange crystals (1OC, 578 nm, ΦF = 50.6%), red crystals (1RC, 613 nm, ΦF = 78.4%) and deep-red emissive amorphous solids (1Am, 667 nm, ΦF = 30.4%) with notable differences in their emission wavelengths and quantum yields. The DCPE-TPA molecules are encapsulated with a polymeric matrix of Pluronic F-127 to form nanoparticles (NPs) that exhibit intense three-photon fluorescence (3PF) in the deep-red region as well as high stability, good photostability and good biocompatibility. Under a 1560 nm fs laser, the DCPE-TPA NPs are further utilized for in vivo 3PF imaging of the vasculature in the brain of a mouse at various vertical depths, producing a penetration depth of 300 μm with a high spatial resolution of 1.8 μm and a high signal-to-background ratio (SBR) of 14. A high-resolution three-dimensional (3D) model of the vasculature beneath the skull could be reconstructed. It is worth mentioning that such a high quantum yield for the deep-red emission of a purely organic material has rarely been reported.

Back to tab navigation

Supplementary files

Article information


Submitted
30 Apr 2020
Accepted
18 May 2020
First published
19 May 2020

Mater. Chem. Front., 2020, Accepted Manuscript
Article type
Research Article

Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

H. Tian, D. Li, X. Tang, Y. Zhang, Z. Yang, J. Qian, Y. Q. Dong and M. Han, Mater. Chem. Front., 2020, Accepted Manuscript , DOI: 10.1039/D0QM00284D

Social activity

Search articles by author

Spotlight

Advertisements